Oh god the 3rd panel references... PhD, jumper cables, scalpel, mysterious liquid, synthoil, Tech X, blue baby... Haven't played the game in a while, but I've got 300 hours clocked into my brain.

Gotta say, I'm really enjoying this comic so far, even though I know next to nothing about the binding of Isaac's plot (Except that it's apparently about some grotesque mommy issues).